Catalog Number - 85518-10P

The 85518‑10P Cogent RP C18 ABX™ HPLC Column is engineered for high‑reliability separations under extreme mobile phase conditions, delivering consistent performance across a wide pH range (2–12).

Constructed with a bulky, specially end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and improves peak shape for acidic, basic, hydrophobic, and neutral analytes, including compounds with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding acid/base methods

✅ One of the most popular RP C18 ABX™ columns for routine analytical workflows

100 mm Length – Increased Resolution for Analytical Methods

• Higher resolving power than 50 mm columns

• Improves separation of closely related compounds

• Increases peak capacity for complex mixtures

• Ideal for method development and refinement

✅ Selected when greater resolution is needed beyond fast screening methods

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • routine QC analysis

  • method development and optimization

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust, reproducible analytical performance

5 µm Particle – Maximum Durability & Reproducibility

• Provides:

  • Long column lifetime

  • Excellent robustness under routine use

  • Lower backpressure vs smaller particle sizes

✅ Ideal for:

• Routine analytical workflows

• Method transfer across labs and instruments

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capabilit

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains stability under aggressive analytical environments

✅ Enables separations beyond standard C18 column limitations

Why Choose RP C18 ABX™ Columns (5 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for stability‑indicating and harsh methods

Maximum Reliability & Method Transferability

• Designed for consistent results across systems

• Ideal for validated and routine methods

Versatile Analytical Performance

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

✅ Trusted choice for routine chromatography and validated QC methods

Application Performance Advantages

• Enables:

  • Reliable retention and resolution

  • Improved performance over legacy 5 µm columns

• Comparable performance to other high‑pH C18 columns used in demanding analytical workflows

✅ Ideal for method optimization and long‑term analytical consistency

System & Sample Considerations

• Compatible with standard HPLC systems

• Suitable for routine and high‑throughput workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain long‑term performance and reproducibility

Typical Applications

• HPLC analytical separations

• Acidic and basic compound analysis

• Pharmaceutical QC and validation

• Stability‑indicating methods

• Hydrophobic and neutral compound separations

• Routine analytical workflows

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for routine and reproducibility‑focused workflows

• Monitor pressure relative to instrument capabilities